Rapid Parallel Screening for Strain Optimization

Progress has been made on identifying biosensors that will be used to report on the fermentation yields of industrially relevant biological compounds. Screening of the desired chemicals has been completed. Microbes that can utilize these compounds as their sole carbon source have been isolated and archived. Genomic DNA from 97 isolates has been prepared for sequencing. Sixty-nine samples are currently in the sequencing queue but were delayed by about a month because the manufacturer had backordered library construction kits. These materials have now arrived and sequence should be available within one month. Sponsored by Defense Advanced Research Projects Agency Microsystems Technology Office (MTO) Program: Living Foundries: Advanced Tools and Capabilities for Generalizable Platforms (ATCG) Issued by DARPA/CMO under Contract No: HR0011-12-C-0062 The views expressed are those of the author and do not reflect the official policy or position of the Department of Defense or the U.S. Government. Approved for Release; Distribution is Unlimited Rapid parallel screening for strain optimization (HR0011-12-C-0062) 2 Table of